A focal adhesion protein-based mechanochemical checkpoint regulates cleft progression during branching morphogenesis

A focal adhesion protein-based mechanochemical checkpoint regulates cleft progression during branching morphogenesis
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  A focal adhesion protein-based mechanochemical checkpointregulates cleft progression during branching morphogenesis William P. Daley 1,2 , Joshua M. Kohn 2 , and Melinda Larsen 2,3 1 Graduate program in Molecular, Cellular, Developmental, and Neural Biology, University atAlbany, State University of New York, Albany, NY 2 Department of Biological Sciences, University at Albany, State University of New York, Albany,NY Abstract Cleft formation is the initial step of branching morphogenesis in many organs. We previouslydemonstrated that ROCK 1 regulates a non-muscle myosin II-dependent mechanochemicalcheckpoint to transition initiated clefts to progressing clefts in developing submandibular salivaryglands. Here, we report that ROCK-mediated integrin activation and subsequent formation of focal adhesion complexes comprise this mechanochemical checkpoint. Inhibition of ROCK1 andnon-muscle myosin II activity decreased integrin  1 activation in the cleft region and interferedwith localization and activation of focal adhesion complex proteins, such as focal adhesion kinase(FAK). Inhibition of FAK activity also prevented cleft progression, by disrupting recruitment of the focal adhesion proteins talin and vinculin and subsequent fibronectin assembly in the cleftregion while decreasing ERK1/2 activation. These results demonstrate that inside-out integrinsignaling leading to a localized recruitment of active FAK-containing focal adhesion proteincomplexes generates a mechanochemical checkpoint that facilitates progression of branchingmorphogenesis. Keywords branching morphogenesis; ROCK; MLC2; fibronectin; focal adhesions; integrin activation INTRODUCTION During morphogenesis, the cells comprising epithelial tissues must be dynamicallyremodeled to build a functional epithelial architecture. In the developing submandibularsalivary gland (SMG), remodeling occurs through the formation of clefts, or indentations, inthe basement membrane surrounding the periphery of an epithelial bud, which then progress,or extend, to separate the epithelium into distinct epithelial lobules. While many hypotheseshave been proposed to explain cleft formation (Spooner and Wessells, 1972; Nakanishi etal., 1987; Nakanishi et al., 1988; Wan et al., 2008), recent evidence suggests that thisprocess is driven by the focal loss of epithelial cell-cell adhesions and their subsequentreplacement by cell-matrix adhesions (Harunaga et al., 2011). However, the mechanisms bywhich such cell-matrix adhesions can promote the physical changes leading to cleftformation remain unknown. 3 Corresponding Author: Melinda Larsen, University at Albany, SUNY, Department of Biological Sciences, 1400 Washington Ave.,LSRB 1086, Albany, NY 12222 USA, Office: 518-591-8882, Lab: 518-591-8887, FAX: 518-442-4767, NIH Public Access Author Manuscript Dev Dyn  . Author manuscript; available in PMC 2013 May 08. Published in final edited form as: Dev Dyn  . 2011 September ; 240(9): 2069–2083. doi:10.1002/dvdy.22714. N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t    The ECM protein fibronectin (FN) is required for cleft formation during SMG branchingmorphogenesis (Sakai et al., 2003; Larsen et al., 2006; Liu et al., 2010). FN is focallyexpressed by epithelial cells adjacent to a forming cleft, and siRNA knockdown of FNprevents cleft formation in embryonic day 12 (E12) SMG organ cultures, while the additionof exogenous FN enhances epithelial clefting (Sakai et al., 2003). Subsequent experimentswith salivary epithelial cells in culture suggested a mechanism by which FN might promotecleft formation; when exogenous FN was added to epithelial cell monolayers, E-cadherin-mediated cell-cell adhesions were rapidly depleted and replaced by FN-mediated cell-matrixadhesions (Sakai et al., 2003). Live imaging of GFP-labeled salivary gland organ explantscultured with fluorescently labeled FN subsequently revealed that FN is directionallytranslocated inward into progressing clefts (Larsen et al., 2006). These studies led to a modelin which FN serves as an ECM “wedge” that promotes the separation of SMG epithelialcells, which, due to the dynamic and plastic nature of the SMG epithelium, reinforces andstabilizes such separation.We recently demonstrated that Rho kinase (ROCK 1)-mediated actomyosin contraction isthe upstream signal that drives FN assembly during SMG cleft progression (Daley et al.,2009). ROCK promotes cytoskeletal contraction by enhancing the activation of myosin lightchain 2 (MLC2), both directly through phosphorylation on Ser19 (Amano et al., 1996;Totsukawa et al., 2000) and indirectly by phosphorylating and inactivating the myosinbinding subunit of myosin light chain phosphatase (Kimura et al., 1996; Feng et al., 1999;Kawano et al., 1999). Such cytoskeletal contraction regulates FN matrix assembly, whichrequires the unfolding of cell surface-bound FN to expose a cryptic self-assembly site viacytoskeletal-mediated forces transmitted through integrin cytoplasmic domains (Zhong etal., 1998; Baneyx et al., 2002; Yoneda et al., 2007).Since ROCK and myosin inhibition prevented cleft progression, but not cleft initiation, ourprevious results suggested that ROCK-stimulated myosin activity is required for thetransition of initiated clefts to a progression-competent state (Daley et al., 2009). Such afinding is consistent with a role for ROCK and myosin in the stabilization of alreadyinitiated clefts, and is supported by earlier observations demonstrating that cleft initiation inthe salivary gland is a dynamic event, with some clefts becoming stabilized at the epithelialsurface, while others rapidly disappear (Nogawa, 1983; Larsen et al., 2006). We proposed amodel for cleft progression in which a localized increase in ROCK-mediated actomyosincontraction reaches a threshold at the epithelial surface that triggers the assembly of FNfibrils in the cleft region, thereby stabilizing the initiated cleft. FN, in turn, promotesepithelial cell proliferation in the regions adjacent to the stabilized cleft and generates anoutward-directed expansion force which results in cleft progression. We designated the stateat which a sufficient threshold of contractility is reached to trigger FN assembly within aninitiated cleft and facilitate its transition to a progression-competent state as amechanochemical checkpoint (Daley et al., 2009). Although the biochemical composition of such a checkpoint was unknown, the requirement for FN assembly in the basementmembrane, which is a cell-mediated process (Zhong et al., 1998; Mao and Schwarzbauer,2005), as well as FN’s role in the downregulation of cell-cell adhesions and theirreplacement by cell-extracellular matrix adhesions (Sakai et al., 2003), suggests that such acheckpoint likely involves an inside-out integrin activation signal that enhances cell-matrixinteractions. Previous studies have demonstrated that the initiation of fibrillogenesis requireshigh-affinity binding of FN to integrins in the activated state prior to fibril assembly (Wu etal., 1995), in further support of an inside-out signal as a key activator of themechanochemical checkpoint.One class of cell-matrix adhesion complexes, known as focal adhesions (FAs), are largecytoplasmic signaling complexes that mechanically link cells to the ECM via cell surface Daley et al.Page 2 Dev Dyn  . Author manuscript; available in PMC 2013 May 08. N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t    integrin receptors. FAs form upon integrin ligation to the ECM, followed by subsequentintegrin clustering at the cell surface to activate the integrin. Such clustering rapidly recruitsmany signaling and scaffolding proteins, examples of which include the adaptor proteinsvinculin, talin, and paxillin, that link integrin cytoplasmic domains to the actin cytoskeleton(Geiger and Bershadsky, 2001; Zaidel-Bar et al., 2003; Wozniak et al., 2004; Bershadsky etal., 2006). A growing body of evidence clearly demonstrates that FAs can form and matureonly if they experience forces transmitted through the actin cytoskeleton. Indeed, the FAprotein talin, which binds directly to integrin cytoplasmic tails and promotes their inside-outactivation (Tadokoro et al., 2003; Tanentzapf and Brown, 2006; Wegener et al., 2007), hasrecently emerged as a potential force sensor that, upon its unfolding by cytoskeletal-mediated forces, promotes FA enlargement by recruiting vinculin to stabilize the adhesion(del Rio et al., 2009). Thus, FA formation is dependent upon non-muscle myosin type IIactivity (Galbraith et al., 2002; Choi et al., 2008), and Rho-mediated contractility has beendirectly implicated in the formation and maturation of such cell-matrix contacts (Ridley andHall, 1992; Chrzanowska-Wodnicka and Burridge, 1996), acting through the Rho-associatedkinase, ROCK.In the current study, we identify molecular components of the mechanochemical checkpoint.We identify FAK as a downstream mediator of a ROCK-mediated actomyosin contractioninside-out integrin activation signal. Importantly, signaling through FAK is required for cleftprogression, but not cleft initiation, and failure of clefts to progress in the presence of FAKinhibition is due to decreased FN assembly and focal adhesion protein localization. FAK isalso required for activation of ERK1/2 to control cell proliferation during cleft progression.These results identify inside-out integrin signaling leading to a localized recruitment of active FAK-containing focal adhesion protein complexes as the molecular componentsdriving progression through the mechanochemical checkpoint to promote FN assembly andcell proliferation to transition initiated clefts to progressing clefts. Finally, we demonstratethat FN assembly itself induces FA protein accumulation and FAK activation in the cleftregion, suggesting that outside-in signaling triggered by FN assembly then acts a signal tostimulate continued cleft propagation downstream of the mechanochemical checkpoint. RESULTS ROCK-mediated actomyosin contraction is an inside-out signal that promotes β 1 integrinactivation and FA formation in the cleft region of E13 SMGs To specifically examine a role for ROCK-mediated actomyosin contraction in the inside-outactivation of  1 integrin in SMG epithelium, we treated mesenchyme-free epithelialrudiments with pharmacological inhibitors of ROCK or non-muscle myosin II andimmunostained them to detect active and total  1 integrin. The monoclonal antibody 9EG7has been reported to selectively bind  1 integrins only when they are in their extended, oractive, conformation (Supplementary Fig. 1A). 9EG7 binding is induced by Mn 2+ , a strongstimulator of  1 function, as well as by FN and RGD peptides (Bazzoni et al., 1995). Theratio of 9EG7 staining intensity to total  1 serves as a measure of the overall level of  1integrins in the activated conformation. To ensure that 9EG7 does indeed bind activated  1integrin in SMG organ cultures, we treated E13 SMGs with increasing concentrations of MnCl 2 . As expected, we observed a dose-dependent increase in the ratio of active to total  1with increasing concentrations of MnCl 2 . MgCl 2 , which does not activate  1 integrin(Bazzoni et al., 1995), did not produce these effects (Supplementary Fig. 1B). Thus, aspreviously reported for cells in two dimensional culture, Mn 2+  treatment induces the 9EG7epitope and results in an increase in activated  1 integrin in SMG ex vivo   organ cultures.There was no change in total  1 integrin expression in ROCK and myosin-inhibited SMGs,as determined by immunoblot analysis (Fig. 1A); however, we observed significant changesin 9EG7 immunostaining. In vehicle control-treated rudiments, 9EG7 staining was restricted Daley et al.Page 3 Dev Dyn  . Author manuscript; available in PMC 2013 May 08. N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t    primarily to the periphery of the epithelium (Fig. 1B), while total  1 was present throughoutthe epithelium but with preferential localization at the epithelial basal periphery. In contrast,ROCK and non-muscle myosin II inhibition resulted in a significant reduction in the ratio of 9EG7 to total  1, with treated SMGs exhibiting only remnant puncta of 9EG7 staining (Fig1B). While 9EG7 staining was particularly pronounced in the progressing clefts of controlintact SMGs, such staining was absent from the immature initiated clefts of ROCK-inhibitedSMGs (Fig. 1B). We conclude that ROCK and myosin-mediated cytoskeletal contraction atthe epithelial surface may constitute an inside-out signal that promotes  1 integrin activationduring SMG branching morphogenesis.Given that ROCK-mediated contractility is capable of activating  1 integrins at theepithelial surface, which is a prerequisite for focal adhesion (FA) complex formation, wenext questioned whether a critical component of the mechanochemical checkpoint might bea ROCK-induced formation of FAs in the cleft region of embryonic SMGs. Since FAs werefirst reported in 2D cell cultures grown on tissue culture plastic, we examined FAs in thesalivary gland epithelial cell line, SCA-9. In vehicle control-treated SCA-9 cell cultures,short stitches of vinculin were present in focal accumulations across the basal surface, wherethey co-aligned with actin stress fibers (Fig. 2A). Accumulations of talin and paxillin, twoother components of focal adhesions, were also observed to associate with these structures(data not shown), suggesting that they are FAs. As previously reported in cultured cells(Galbraith et al., 2002), ROCK and myosin inhibition prevented the formation of such cell-matrix adhesions and disrupted F-actin organization (Fig. 2A). We observed similar resultswhen we stained for paxillin and talin, in that FAs were not observed (data not shown),indicating that ROCK and myosin are required for FA formation in salivary epithelial cellsin 2D cell culture.We next examined FA protein levels and localization in E13 organ explants cultured in thepresence of the ROCK and myosin inhibitors. While we observed no significant differencein the levels of the FA proteins, vinculin and paxillin, there was an approximately 50%reduction in the levels of talin protein in the presence of ROCK and myosin inhibitors (Fig.2B). Immunocytochemistry for FA proteins in intact E13 SMGs revealed that in vehiclecontrol SMGs, both vinculin and talin were detectable at low levels within the interior of theepithelium at cell borders, but they localized much more intensely at sites of cell-matrixcontacts adjacent to the basement membrane, as indicated by co-staining with an antibodydetecting the basement membrane protein, collagen IV (Fig. 2C, D). Moreover, suchstaining was particularly intense in the distal cleft regions (Fig. 2C, D). In contrast, vinculinand talin staining were greatly reduced adjacent to the basement membrane and in the cleftregions of ROCK and myosin-inhibited SMGs. These results demonstrate that when ROCK-mediated actomyosin contraction is perturbed, FA protein localization within cleft regions iscompromised. Since these clefts are also blocked at the initiation stage, these results alsosuggest that such FA proteins are components of the mechanochemical checkpoint thatstabilizes initiated clefts and promotes their transition to a progression-competent state. Focal adhesion kinase (FAK) activation occurs downstream of ROCK-mediatedactomyosin contraction during SMG branching morphogenesis Focal adhesion kinase (FAK) is a non-receptor protein tyrosine kinase that localizes to FAsin a variety of tissues and is a crucial mediator of downstream signals from cell-matrixadhesions (Parsons et al., 2000; Tilghman and Parsons, 2008; Zhao and Guan, 2009). Theclustering of integrins upon adhesion to the ECM leads to the rapid recruitment andactivation of FAK at FAs, primarily due to phosphorylation on Tyr397 by adjacent FAKmolecules, with subsequent phosphorylation on Tyr576/577 required for maximal FAKactivation (Lipfert et al., 1992; Schaller et al., 1994; Calalb et al., 1995). Active FAKmaintains and further promotes the generation of cellular tension in response to adhesion to Daley et al.Page 4 Dev Dyn  . Author manuscript; available in PMC 2013 May 08. N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t    the ECM through maturation and strengthening of FAs (Michael et al., 2009). Furthermore,Rho-mediated contractility through its downstream effector ROCK has been implicated inthe stretch-induced activation of FAK at Tyr397 (Seufferlein and Rozengurt, 1994; Torsoniet al., 2005).Since signaling through FAK is involved in FA strengthening and maturation, we questionedwhether FAK might function as part of the mechanochemical checkpoint downstream of ROCK and myosin. To this end, we performed Western analysis on ROCK and myosin-inhibited SMG cell lysates with antibodies specific for the phosphorylated forms of FAK,and observed a dose-dependent decrease in FAK phosphorylation on Tyr397 (pFAK Y397)and on Tyr576/577 (pFAK Y576/77) (Fig. 3A). We performed immunocytochemistry toexamine the localization of pFAK Y397 in SMG epithelial rudiments. In vehicle control-treated rudiments, pFAK Y397 localized to the extreme periphery of epithelial buds and co-localized with the FA protein talin (Fig. 3B). Such staining and localization weresignificantly decreased in ROCK and myosin-inhibited rudiments (Fig. 3B). These resultsdemonstrate that ROCK-mediated actomyosin contraction is required for thephosphorylation and activation of FAK in SMGs undergoing branching morphogenesis.Since ROCK signaling also promotes  1 integrin activation and FA formation at theperiphery of epithelial buds, it is likely that these effects on FAK activation are caused byROCK-mediated integrin clustering at the epithelial surface and in cleft regions. Inhibition of FAK blocks SMG branching morphogenesis at the cleft initiation stage andpromotes additional cleft initiations We investigated a function for FAK in cleft progression by culturing E13 SMGs in thepresence of two structurally distinct pharmacological inhibitors of FAK, FAK14 andPF-573228, as well as FAK siRNAs. With both the FAK inhibitors and with FAK siRNAs,we observed a dose-dependent inhibition of branching (Fig. 4A). Furthermore, themorphology of SMGs lacking FAK function strongly resembled that of SMGs that had beentreated with FN siRNA, suggesting a functional link between FN and FAK signaling.Interestingly, FAK inhibition also resulted in an increase in the number of small initiated,but not elongated, clefts at the surface of SMG epithelial buds, much like the immatureinitiated clefts resulting from treatment with ROCK and myosin inhibitors (Fig. 4B)previously reported (Daley et al., 2009). Morphometric analysis of SMGs treated withFAK14, PF-573228, and FAK siRNA revealed a dose-dependent decrease in the number of completed epithelial buds and an increase in such small initiated clefts (Fig. 4B). Consistentwith these results, FA protein accumulation in the cleft region was likewise impaired inFAK-inhibited SMGs, suggestive of a role for FAK in the stabilization of initiated clefts viathe formation of cell-matrix adhesions (Fig. 4C). To confirm the effectiveness of FAKinhibition and knock-down, we performed Western analysis on SMG cell lysates treatedwith PF-573228 and FAK siRNA. We observed a dose-dependent decrease in both pFAKY397 and pFAK Y576/577 with increasing concentrations of PF-573228 and a decrease intotal FAK protein levels with FAK siRNA (Fig. 4D). Taken together, these results indicatethat FAK activation, downstream of ROCK and non-muscle myosin II activation, is acritical regulator of cleft progression during SMG branching morphogenesis and is requiredfor the transition of initiated clefts to progressing clefts. Inhibition of FAK activity disrupts FN assembly in the basement membrane of SMG organcultures and decreases cell proliferation Since we previously demonstrated that FN assembly drives subsequent cleft progressiononce the mechanochemical checkpoint has been reached, we questioned whether FAKactivation might also be required for FN assembly in SMG basement membranes. Wetherefore compared total endogenous FN expression by immunoblotting in the presence of  Daley et al.Page 5 Dev Dyn  . Author manuscript; available in PMC 2013 May 08. N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  N I  H -P A A  u t  h  or M an u s  c r i   p t  
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